Fig. 1.

Lphn1-deficient mice display an increased weight and fat accumulation. a Lphn1 knockout (KO) mice are bigger than wild-type (WT) littermates. Representative images of 30-week-old female mice on a cm scale. b Lphn1 knockout mice gain more weight over time than wild-type littermates. Mice were weighed weekly. Weight differences become visible after 12 weeks in females as well as males and increase with age. Data are given as means ± SD; n ≥ 8; *p < 0.05; **p < 0.01; ***p < 0.001. c The body length of Lphn1 knockout mice is indifferent from the one of wild-type littermates, but tails are shorter. Body and tail lengths were measured in mice 30–34 weeks of age. Given are means ± SD; n = 5-6; ***p < 0.001. d Lphn1 knockout mice have more fat, but similar lean body mass. Body composition of 30–34-week-old mice was determined using EchoMRI. Given are means ± SD; n ≥ 10; **p < 0.01; ***p < 0.001. e Organ mass of Lphn1 knockout and wild-type mice are similar. Only the livers are significantly heavier in Lphn1 knockout animals (30–34-weeks of age). Data are means ± SD; n ≥ 8; **p < 0.01. f Representative Oil red O staining of liver sections of 30–34-week-old male mice used to directly visualize the stored triglycerides in liver cells (red). The staining reveals high numbers of large lipid droplets within Lphn1 knockout livers. g Livers of Lphn1 knockout mice contain more fat than wild-type livers. Liver fat and lean body mass of 30–34-week-old animals was determined by EchoMRI. Data are given as means ± SD; n ≥ 5; **p < 0.01; ***p < 0.001