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. 2024 Apr 25;10:193. doi: 10.1038/s41420-024-01946-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A The Venn diagram shows the intersection of three sets: 48 VM-associated genes, 2886 predicted miR-134-5p target genes, and 7873 DEGs. B Heatmap showing expression of seven genes between OS and control tissue in the GTEx-TARGET cohort. C KEGG enrichment analysis of the seven candidate miR-134-5p target genes. D Protein levels of p-PI3K, PI3K, p-Akt, and Akt in OS cells following transfection with the miR-134-5p mimic or miR-134-5p inhibitor or the corresponding negative control. E, F mRNA expression of seven candidate genes in cells transfected with the miR-134-5p mimic. G Protein and mRNA levels of MMP2 and ITGB1 in miR-134-5p mimic-transfected OS cells versus control. H Protein and mRNA levels of MMP2 and ITGB1 in miR-134-5p-knockdown OS cells versus control. I Protein and mRNA levels of MMP2 and ITGB1 in OS cells versus osteoblasts. Statistics using one-way ANOVA with Tukey’s test. J, K Protein and mRNA levels of MMP2 and ITGB1 in OS tissues versus normal bone. Statistics using independent Student’s t-test. L Wild-type and mutant binding sites for ITGB1 in miR-134-5p. M Luciferase assay of HOS cells transfected with miR-134-5p mimic (10 nM) or control miR-NC and plasmids containing 3′ UTRs of ITGB1. Statistics using one-way ANOVA with Tukey’s test. The data represent means ± SD. ns not significant; *p < 0.05; **p < 0.01; ***p < 0.001.