Fig. 4. Numb is required for Shh-induced Ptch1 exit from the cilium.

a Western blot analysis showing the absence of Numb protein in the Numb CRISPR/Cas9 knockout cell clones. Experiments were performed three times with similar results. b Wild type (WT) or Numb KO cells were pre-treated with SAG for 24 h to induce Ptch1 expression and cilium accumulation, followed by Shh stimulation for 1 h, and subsequently fixed for immunofluorescence staining. The cilia were marked by acetylated-tubulin (red). c Quantification of Ptch1 intensity in the cilium. Data are shown as mean ± SD. A total of 87 cells were quantified for each experimental condition. Statistics: Two-way ANOVA with multiple comparisons (Tukey test). ns not significant. Source data are provided as a Source Data file.