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Immunoprecipitation with MT.M MAbs from lysates of vectorially iodinated MT2 cells. MT2 cells were surface labeled with 125I, and immunoprecipitation analysis was carried out as described in Materials and Methods. In this experiment, the cell lysate was precleared with normal rabbit serum and SaC to remove nonspecific binding proteins. Antibody controls consisted of myeloma control (IgG1), anti-CD54 (MT.M5), anti-CD44 (U9.M2), anti-CD18 (H52), and anti-class II MHC (MHM.36 and H53).
