TABLE 2.
MAb | MT2
|
MJ
|
K562/VCAM-1
|
|||
---|---|---|---|---|---|---|
% Pos | MCF | % Pos | MCF | % Pos | MCF | |
H53 | 99.8 | 976 | 99.9 | 949 | 1.4 | 14 |
MT.M1 | 99.7 | 768 | 99.9 | 672 | 0.3 | 15 |
MT.M2 | 99.3 | 876 | 99.9 | 921 | 9.6 | 19 |
MT.M3 | 99.4 | 893 | 99.9 | 985 | 8.0 | 19 |
MT.M4 | 99.6 | 948 | 99.8 | 887 | 5.8 | 18 |
H5C6 | 89.4 | 21 | 99.8 | 39 | 94.2 | 62 |
MHM.5 | 99.3 | 923 | 99.9 | 441 | 0.9 | 14 |
The indicated MAbs were tested for binding to MJ, MT2, and K562/VCAM-1 cells by flow cytometry as described in Materials and Methods. Data shown are percent positive cells relative to an isotype-matched myeloma IgG (% Pos) and the intensity of staining, shown as MCF. MAbs H53, H5C6, and MHM.5 recognize class II MHC, CD63, and class I MHC, respectively.