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. 2024 Apr 25;15:3490. doi: 10.1038/s41467-024-47935-9

Fig. 4. XPA-dependent prolonged TFIIH-DNA damage binding impairs neuron functionality.

Fig. 4

A AID::GFP::GTF-2H1 recruitment to oocyte chromosomes, 15 and 30 min after UV-B-irradiation of living wild-type, xpa-1, xpf-1 or xpa-1;xpf-1 animals. Scale bar, 10 µm. B AID::GFP::GTF-2H1 recruitment in fixed animals. Mean with SEM of two independent experiments. Numbers represent p-values (ANOVA corrected for multiple comparisons). n (left to right)= 110, 115, 106, 117, 129, 99, 94, 102 bivalents. C Dil-dye filling of wild-type, xpf-1, xpg-1, xpa-1, xpa-1; xpf-1 and xpg-1 xpa-1 animals, 72 h after UV-B irradiation. Scale bar, 10 µm. Fraction of dye filling animals 72 h after increasing UV-B dose for (D) wild-type, xpf-1, xpa-1 and xpa-1; xpf-1 or (E) wild-type, xpg-1, xpa-1 and xpg-1 xpa-1. Mean with SEM of three independent experiments. Numbers represent p-values (ANOVA corrected for multiple comparisons), comparing double mutant to xpf-1 or xpg-1. n (left to right)= (D) 62, 57, 55, 60, 70, 45, 49, 48, 61, 23, 45, 21, 57, 40, 38, 41; (E) 64, 57, 81, 70, 73, 46, 44, 62, 62, 26, 46, 59, 58, 33, 51, 52. L1 larvae survival of (F) wild-type, xpf-1, xpa-1 and xpa; xpf-1 or (G) wild-type, xpg-1, xpa-1 and xpg-1 xpa-1 animals. Mean with SEM of three independent experiments. Numbers represent p-values (ANOVA corrected for multiple comparisons), comparing xpf-1 or xpg-1 to double mutant. n (left to right)= (F) WT:853, 865, 874, 822; xpf-1:351, 334, 337, 229; xpa-1:1326, 1671, 1564, 1027; xpa-1;xpf-1:743, 668, 702, 521; (G) WT:853, 865, 874, 822; xpg-1:238, 220, 113, 104; xpa-1:1326, 1671, 1564, 1027; xpg-1 xpa-1:1389, 1475, 1177, 752. H Fraction of dye filling wild-type and xpf-1(tm2842), (emc98[D719A]) and (emc105[R805W]), 72 h after 40 J/m2 UV-B-irradiation. Mean with SEM of three independent experiments. Numbers represent p-values (ANOVA corrected for multiple comparisons), compared to wild-type. n (left to right)= 135, 88, 150, 150, 134, 104, 137, 150. I Scheme of dye filling assay indicating time periods of AID::GFP::GTF-2H1 depletion. J Fraction of dye filling animals with AID::GFP::GTF-2H1 depleted as in (I). Mean with SEM of three independent experiments. Numbers represent p-values (ANOVA corrected for multiple comparisons). n (left to right)= 55, 30, 47, 52, 45, 45, 48, 31, 37, 32. Source data are provided as a Source Data file.