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. 2024 Apr 25;15:3528. doi: 10.1038/s41467-024-47847-8

Fig. 8. BIN1 knockdown does not recover age-related diastolic dysfunction.

Fig. 8

a, b Representative pulsed-wave (PW) Doppler images from unconscious young (a) and old (b) mice before and two, four, and six weeks after RO-injection of shRNA-scrmb (left) and shRNA-mBIN1 (right). Summary dot-plots for young (N = 10) and old (N = 19) mice, paired results before and two weeks after RO-injection of old mice with shRNA-scrmb (N = 10) and shRNA-mBIN1 (N = 9), and paired results before and two, four and six weeks after RO-injection of old mice with shRNA-scrmb (N = 4) and shRNA-mBIN1 (N = 4) for the following diastolic measurements are displayed: c–e isovolumetric relaxation time (IVRT) and, f–h mitral valve E/A ratio (MV E/A). Statistical analysis was performed on data in (c, f ) using unpaired two-tailed Student’s t-tests, on data in (d, g) using paired two-tailed Student’s t-tests, and on data in (e, h) using one-way ANOVAs with multiple comparison post-hoc tests. Young data are from NIA young mice. Data are presented as mean ± SEM. Source data are provided in the Source Data file.