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. 2024 Apr 25;10:191. doi: 10.1038/s41420-024-01966-9

Fig. 3. Analysis of the efficacy of the selected NLRP3 inflammasome inhibitors in human THP-1 macrophages expressing SARS-CoV-2 viroporins.

Fig. 3

SARS-CoV-2 proteins ORF3a, E and M were expressed in THP-1-ASC-GFP cell line to trace inflammasome assembly and pyroptosis in the presence of selected inhibitors of the inflammasome: MCC950, colchicine, disulfiram and KCl. A Time-lapse analysis of ASC-speck formation. B Time-lapse analysis of cell death kinetics. C Level of cell death assessed by LDH secretion 24 h after lentiviral infection. D IL-1β secretion 24 h after lentiviral infection. Graphs represent means ± SEM, n = 3–5, multiple comparisons were performed with Kruskall-Wallis test. p < 0.05 vs control is marked: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.