Figure 3.

Mineralization in vitro. (A–D) Representative bright field images of normal (A), and FAM20C RNS (B,C) deficient GFs grown for 21 days in standard medium colored with Alizarin Red. (D–F) Representative bright field images of normal (D) and FAM20C (E,F) deficient GFs grown for 21 days in a mineralization-inducing medium colored with Red Alizarin. Arrowheads indicate rare and small calcium deposits in controls GFs. (I) Quantitative analysis of Alizarin Red expression by morphometric analysis on the pooled results of three separate experiments; the value represents the means ± SD. ***p < 0.001. Scale bars: (A–F) = 20 μm.