Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
DROPPS workflow for low-input protein digestion
Cells are deposited onto Teflon-coated slides by fluorescence-activated cell sorting or standard pipette. Cell transfer buffer is evaporated, and slides can be optionally stored at −80°C. Cell lysis and protein digestion are performed with MS-compatible reagents in a simple humidity chamber. Samples are transferred to standard sample vials or plates and analyzed by LC-MS for quantitative proteomic analysis.
