TABLE 2.

Intra- and intersample variability of HVR-1 during HCV primary infection

Sample	Intrasample variability		Samples	Intersample variability
	Ka/Ksa	gdb (mean)		rKa/rKsa	gdb (mean ± SD)
A1	0.87	3.67
A2	0.66	7.56	A1-A2	0.68	6.51 ± 2.66
A3	0.00	0.00	A1-A3	0.56	6.90 ± 2.45
A4	2.47	2.47	A1-A4	0.69	5.14 ± 2.92
B1	2.80	2.11
B2	1.26	5.88	B1-B2	3.00	1.47 ± 0.89
B3	4.90	0.35	B1-B3	2.02	4.40 ± 1.92
B4	2.16	13.78	B1-B4	2.88	8.24 ± 2.96
C1	1.42	7.79
C2	1.69	4.80	C1-C2	1.57	6.06 ± 2.58
C3	1.11	10.83	C1-C3	0.51	32.53 ± 11.65

^aThe average number of nucleotide substitutions per nonsynonymous site and per synonymous site for all pairwise comparisons within each sampling point (K_a, K_s) and of sequences obtained at different time points (rK_a, rK_s) were calculated by using the Jukes-Cantor model of molecular evolution implemented in the MEGA program (see Materials and Methods). 

^bThe intra- and intersample gd were calculated with DNADIST software implemented in the PHYLIP package (version 3.5c).