FIG. 7.

Detection of the desmin filament network and viral capsid antigens in infected BHK-21 cells by double-immunofluorescence confocal microscopy. Infected cells were incubated with the anti-VP1 MAb and a polyclonal anti-desmin antibody, D8281. After being washed, the cells were incubated with a TRITC-labeled rabbit anti-mouse Ig antibody and with an FITC-labeled rabbit anti-goat Ig antibody as described in Materials and Methods. (A) DA-infected cells, 5 h p.i. (B) GDVII-infected cells, 5 h p.i. (C) DA-infected cells, 8 h p.i. Upper panels: virus (red) and desmin (green). Lower left panel: combined image of red and green fluorescence. Lower right panel: pixels of high intensity for both colors. (D) GDVII-infected cells, 8 h p.i. Upper panels: virus (red) and desmin (green). Lower left panel: combined image of red and green fluorescence superimposed on the pixels of high intensity for both colors (white). Lower right panel: cytofluorogram analysis.