Fig. 2.

Maternal obesity predisposes to the development of steatosis and fibrosis in adult life

(A) Representative H&E-stained histological liver sections from different time points for HFD and ND offspring. (B) Automated quantitative analysis of steatotic tissue was performed. (C) Evolution of steatosis over time. (D) Representative Masson-Trichrome-stained histological liver sections from different time points for HFD and ND offspring. (E) Automated quantitative analysis of fibrotic tissue was performed. (F) Expression of fibrosis markers in liver tissue of female offspring at 40 weeks. Data presented as median ± IQR (B, E, F) or mean ± SE (C) one dot represents one animal (B, E, F), level of significance of p = 0.05. Statistical analysis was performed by Wilcoxon–Mann–Whitney test. ND_Fem: Female offspring born to lean mothers n = 6-15, F_HFD: Female offspring born to obese mothers n = 6-10, M_ND male offspring born to lean mothers n = 5-13, M_HFD male offspring born to obese mothers n = 5-14. Scale bar Fig. 2A and 2D: 100 μm. HFD, high-fat diet; ND, normal diet.