Fig. 3.

Maternal obesity promotes liver inflammation

(A) Representative H&E-stained histological liver sections with immune cell infiltrates. (B) Automated quantitative analysis of tissue surface covered by immune infiltrates. Representative immunofluorescence liver sections of female offspring with corresponding automated quantification (C) CD45, (D) Iba1 and (E) CD8 staining. (F) Expression of TLR family members TLR2 and TLR4 in liver tissue of female offspring at 40 weeks. (G) Expression of inflammatory markers in liver tissue of female offspring at 40 weeks. Data presented as median ± IQR, one dot represents one animal, level of significance of p = 0.05. Statistical analysis was performed by Wilcoxon–Mann–Whitney test. Statistical analysis: Wilcoxon signed-rank test (B-G). Median ± IQR and mean ± SE for panel F and G. ND_Fem: Female offspring born to lean mothers n = 6-15, F_HFD: Female offspring born to obese mothers n = 6-10, M_ND male offspring born to lean mothers n = 5-13, M_HFD male offspring born to obese mothers n = 5-14. Scale bar Fig. 2A: 100 μm, 2C-E: 50 μm. HFD, high-fat diet; ND, normal diet.