(A) Schematic of iron diet treatment 350ppm and <5ppm in Hif2αfl/fl and Hif2αΔmφ mice to induce iron deficiency anemia. (B) Serum iron measured in 12 week old Hif2αfl/fl and Hif2αΔmφ mice 2 week post dietary intervention (C) Analysis of RBC, MCV, HB, and HCT from 12 week old Hif2αfl/fl and Hif2αΔmφ (n = 7–9 per group). (D) Serum iron measured in 8 month old Hif2αfl/fl and Hif2αΔmφ mice 2 week post dietary intervention. (E) Analysis of RBC, MCV, HB, and HCT from 8 month old Hif2αfl/fl and Hif2αΔmφ (n = 7–9 per group). Data represent the mean ± SEM. Significance was determined by unpaired t test. *P < 0.05, ***P < 0.001, and ****P < 0.0001 versus the Hif2αfl/fl mice fed with 350ppm iron containing diet.