qPCR analysis of A) Liver Hepcidin, B) Renal Erythropoietin and C) duodenal Slc11a2 (Dmt1), (D)
DcytB, (E)
Slc40a1 (Fpn) and (F)
Tfrc from mice fed 350ppm or 5ppm iron containing diet for 2 weeks (n=4-5 per group). Data represent the mean ± SEM. Significance was determined by unpaired t test. *P < 0.05, ***P < 0.001, and ****P < 0.0001 versus the Hif2αfl/fl mice fed with 350ppm iron containing diet.