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Effect of mutation of the CRS on cumulative CAT expression from the R1 silencer-containing US3 promoter at various times after infection. HFF cells were infected with approximately 5 PFU of RVgptR1CAT or RVgptR1crs−CAT per ml, and the amount of CAT activity per microgram of protein was determined at various times after infection as described in Materials and Methods.
