FIG. 7.

Effect of a CRS mutation in a nonpermissive cell type. Undifferentiated THP-1 cells were infected with approximately 3 PFU of either RVgptR₂CAT or RVgptR₂crs⁻CAT per cell. After viral adsorption, the cells were washed in medium and suspended in medium with or without LPS. CAT activity per microgram of protein was determined at various times after infection as described in Materials and Methods. (A) THP-1 cells infected with RVgptR₂CAT; (B) THP-1 cells infected with RVgptR₂crs⁻CAT.