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CAT expression from the US3 promoter in 293-T cells cotransfected with a plasmid expressing either wild-type or mutant IE86. Cells were cotransfected by the calcium phosphate precipitation method and analyzed for CAT activity and for IE86 or IE86HL expression as described in Materials and Methods. (A) Autoradiogram of 14C-chloramphenicol and its acetylated derivatives; (B) Western blot of IE86 and IE86HL expression in 293-T cells.
