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Binding of the IE2 gene product to the CRS of the US3 promoter. Wild-type (rIE2) and mutant (rIE2HL) maltose-IE2 fusion proteins were purified as described previously (28, 29) and used for EMSA as described in Materials and Methods. Lanes: 1, wild-type CRS plus 2.9 pmol of rIE2; 2, wild-type CRS plus 5.8 pmol of rIE2; 3, wild-type CRS plus 5.8 pmol of rIE2HL; 4, mutant CRS plus 2.9 pmol of rIE2; 5, mutant CRS plus 5.8 pmol of rIE2; 6, mutant CRS plus 5.8 pmol of rIE2HL.
