FIGURE 2.

(A) RT-qPCR analysis of FMO1 FMO3 and FMO5 transcript levels in the liver of WKY, SHR and WKY-ANG rats (displays on histogram use arbitrary units). (B) FMO1, FMO3 and FMO5 protein levels in the liver examined by Western blot analysis. Beta-actin and the Ponceau-S staining were used as a reference for equal protein loading control. Quantification of the band intensity of protein expression was performed using Quantity One software The relative levels of the test proteins are plotted in arbitrary unit (means ± SD). (C) Representative blots of hepatic FMO’s protein of WKY, SHR and WKY-ANG rats. *p < 0.05 vs. WKY, **p < 0.01 vs. WKY #p < 0.05 SHR vs. WKY-ANG.