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. 2024 Apr 26;22:51. doi: 10.1186/s12958-024-01216-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The apoptosis of ovarian tissue in three groups. (A): TUNEL fluorescence staining of the three groups. Green indicates positive staining areas (100 μm and 50 μm). (B): Statistical histogram of TUNEL staining. (C): The expression of Bax in ovarian tissues of the three groups was detected via IHC. Brown indicates positive staining areas (50 μm). (D): Statistical histogram of Bax by IHC. (E): The expression of Bcl-2 in ovarian tissues of the three groups was detected via IHC. Brown indicates positive staining areas (50 μm). (F): Statistical histogram of Bcl-2 by IHC. (G-H): The expression of Bax in ovarian tissues of the three groups was detected by using Western blotting, and a statistical histogram was conducted (All results were corrected by Bonferroni method, and P < 0.05 after correction was considered to be statistically significant); n ≥ 3