Fig. 5.

Fibrosis-related detection in three groups of mice. (A): Three groups of Masson staining. Blue areas indicate fibrosis (50 μm). (B): Three groups of Sirius red staining, the red area indicate fibrosis. (C-D): IHC detection of α-SMA and collagen I in the three groups (50 μm). (E): Immunofluorescence detection of α-SMA in ovarian tissue of mice in three groups. Green indicates positive areas (100 μm). (F): Statistical histogram of Masson staining. G: Statistical histogram of sirius red staining. H: Statistical histogram of positive staining for Collagen I. (I): Statistical histogram of positive staining for α-SMA. J: Statistical analysis of α-SMA immunofluorescence staining. (All results were corrected by Bonferroni method, and P < 0.05 after correction was considered to be statistically significant); n ≥ 3