Fig. 7.

Correlation detection of ovarian tissue oxidative damage in three groups of mice. (A-C): IHC detection of 8-OHdG, 3-NIT, and 4-HNE (50 μm). The brown area indicates the positivity area. (D): Statistical histogram of positive staining for 8-OHdG (E): Statistical histogram of positive staining for 3-NIT. (F): Statistical histogram of positive staining for 4-HNE. (G-H): WB detection and statistical analysis of NOX4 in ovarian tissue of three groups of mice (All results were corrected by Bonferroni method, and P < 0.05 after correction was considered to be statistically significant); n ≥ 3