Fig. 4.

PXL770 treatment improves neuroinflammation gene expression markers in lymphocytes from a C-ALD patient and in Abcd1 KO mouse glial cells. (A) Lymphocytes were isolated from a C-ALD patient and (B) Mixed glial cells (astrocyte-enriched) were isolated from 1-day-old wt and Abcd1 KO mice and matured for 10 days, then stimulated (TI) with TNFα (10ng/ml) and IL1β (10ng/ml) for 70 hours (during PXL770 treatment). Cells were exposed to PXL770 10 μM for 72 hours and mRNA levels were evaluated by RT-qPCR, normalized by RLP27/Rlp27 expression (no unit). Data are mean ± S.E.M., n = 3 replicates. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 (Dunnett’s or Kruskal Wallis test versus TI untreated – test wt versus TI untreated not shown).