FIG. 2.

Influence of baf on endocytic transport of FITC-dextran as analyzed by fluorescence microscopy. HeLa cells grown on chamber slides were preincubated without or with baf (20 nM or 200 nM) in L15 medium for 30 min at 37°C. Ten milligrams of FITC-dextran per ml was added, and endosomes were labeled for 25 min at 37°C. Cells were cooled, rinsed with PBS, fixed with 4% paraformaldehyde, quenched with 50 mM NH₄Cl in PBS, and viewed with an Olympus fluorescence microscope.