Figure 5.

Validation of association analysis using QTL Interval. (A) LOD curves of QTL mapping for level of n1048 in maize kernels on chromosome 10. (B) Scatterplot of association results between SNPs in the confidence interval and the level of n1048. Association analysis was performed using the mixed linear model controlling for the population structure (Q) and kinship (K). (C) The candidate genes of 200kb in the confidence interval. (D) Box plot for n1048 (red) from AMPE1 and expression of GRMZM2G066142 (sky blue) and GRMZM2G066049 (sky blue). (E) Plot of correlation between the n1048 level in AMPE1 and the normalized expression level of the GRMZM2G066142 and GRMZM2G066049, red triangle and blue spot represented the lead SNP GG and AA, respectively. The r value is based on a Pearson correlation coefficient. The p value is calculated using the Student’s-t test.