TABLE 1.
Efficient removal of HIV-1 envelope-binding Abs from purified Ig from HIV-infected patients
| Samplea | ELISA endpoint antibody titer against:
|
||||
|---|---|---|---|---|---|
| ogp140451 | gp120451 | V3MN | gp41582 | p24 | |
| US20 | |||||
| Purified Ig | 409,600 | 51,200 | 12,800 | 6,400 | 204,800 |
| gp120-depleted fraction | 204,800 (2b) | 400 (128) | 3,200 (4) | 6,400 (1) | 204,800 |
| ogp140-depleted fraction | 1,600 (256) | 400 (128) | 1,600 (8) | <400 (>16) | 204,800 |
| gp120 Abs | 25,600 | 12,800 | 400 | <100 | <100 |
| ogp140 Abs | 51,200 | 12,800 | 400 | 400 | 800 |
| HIVIG | |||||
| Purified Ig | 1,638,400 | 409,600 | 25,600 | 102,400 | 1,600,000 |
| gp120-depleted fraction | 819,200 (2) | 200 (2,048) | 3,200 (8) | 102,400 (1) | 1,600,000 |
| ogp140-depleted fraction | 200 (8,192) | 400 (1,024) | 6,400 (4) | 12,800 (8) | 1,600,000 |
| V3MN-depleted fraction | 819,200 (2) | 409,600 (1) | <100 (>256) | NDc | 1,600,000 |
| gp120 Abs | 102,400 | 102,400 | 3,200 | <800 | 1,600 |
| ogp140 Abs | 409,600 | 102,400 | 3,200 | 25,600 | 3,200 |
a
Purified Ig, purified Ig from HIV-1 serum; gp120-, ogp140-, and V3MN-depleted fractions, HIV-1 sera depleted over gp120, ogp140, and V3MN columns, respectively; gp120 Abs and ogp140 Abs, Abs purified from HIV-1 serum by using gp120 and ogp140 affinity columns, respectively. Values for depleted fractions were normalized to the unfractionated Ig by correcting for the amount of Ab to p24, and affinity-purified (eluted) Abs were concentrated to the original volume of the Ig applied to each column, as described in Materials and Methods.
b
Fold reduction compared to Ig.
c
ND, not determined.