FIG. 2.

Mobility and competition assay of RNAs from PV and HAV 5′NCR. ³²P-labeled PV stem-loop IV RNA (nt 220 to 460) was incubated without (lane 1) and with rPCBP2 (lane 2) and separated on a 4% native polyacrylamide gel. Competition for complex formation between ³²P-labeled PV stem-loop IV RNA and rPCBP2 was tested in the presence of 1- to 40-fold molar excesses of transcripts representing the PV 5′NCR (P1-816; lanes 3 to 7) or transcripts representing the HAV 5′NCR (H1-744; lanes 8 to 12). Free labeled PV stem-loop IV (stIV) RNA and the RNA-protein complex are indicated to the left.