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. 2024 Apr 20;13(4):492. doi: 10.3390/antiox13040492

Figure 1.

Figure 1

Accumulation of red elemental selenium in selenite-treated E. coli bacteria. (A) E. coli OP50 bacteria were grown in DYT medium supplemented with different concentrations of sodium selenite. Bacterial growth was assessed by measuring the optical density (OD) of the E. coli suspensions at 600 nm at the indicated time points. Data are shown as means ± S.E.M. from three independent experiments. (B) Representative photographs, showing aliquots of the selenite-treated E. coli suspensions in cuvettes. (C) Photographs of E. coli OP50 bacteria streaked on LB agar plates without added selenite (left plate) or supplemented with 1 mM selenite (right plate) and grown at 37 °C for 48 h. (D) E. coli OP50 bacteria were grown for 48 h in DYT medium supplemented with 1 mM sodium selenite. Se contents of the growth medium and the bacterial pellet were measured by total reflection X-ray fluorescence spectrometry (TXRF) at the beginning and at the end of the experiment. Data are depicted as means ± S.E.M. from three independent experiments; statistical analysis was performed using a paired t-test (* p < 0.05).