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. 2024 Apr 20;13(4):492. doi: 10.3390/antiox13040492

Figure 2.

Figure 2

Developmental retardation of C. elegans fed a diet of Se-enriched E. coli bacteria. Bristol N2 (wild-type) worms at the L1 stage were supplied with either the standard E. coli OP50 diet or with Se-enriched E. coli OP50 for up to three days after reaching adulthood. Body length (A) and body area (B) of 10 worms from each of the specified consecutive developmental stages were determined by microscopy. Data are depicted as means ± S.E.M. from three independent experiments; statistical analysis was performed using one-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001 vs. L1 worms; # p < 0.05 vs. worms fed on the standard E. coli diet). (C) Representative photographs of nematodes which received the standard E. coli OP50 diet (left panel) or Se-enriched E. coli OP50 (right panel) from the L1 stage until 3 days of adulthood. (D) Larvae produced by C. elegans fed either the standard E. coli OP50 diet or the Se-enriched bacteria. The total (left panel) and daily (right panel) numbers of offspring were measured for up to 10 days after the worms reached the L1 stage. Data are depicted as means ± S.E.M. from four independent experiments; statistical analysis was performed using an unpaired t-test with Welch’s correction (# p < 0.05 vs. worms fed on the standard E. coli diet).