Skip to main content
. 2012 Aug;342(2):461–471. doi: 10.1124/jpet.112.194464

Fig. 7.

Fig. 7.

A, effect of NMDA on aortic NOx content in the absence or presence of the nonselective NOS inhibitor l-NAME (10 mg/kg i.v.) or PE infusion (2.7 μg/kg/min; 18 μl/min). B, effect of NMDA in the absence or presence of the selective nNOS inhibitor NPLA (150 μg/kg i.p.), the selective eNOS inhibitor l-NIO (10 mg/kg i.v.), or the glycine/NMDAR antagonist R-(+)-HA-966 (10 mg/kg i.v.). Values are means ± S.E.M. of four to six observations in these ex vivo studies. C, NO generation in aortic sections measured by DAF-FM fluorescence in aortic sections from untreated rats (n = 10 for each treatment) in the presence of saline (control) (A), NMDA (100 μM) (B), the nonselective NOS inhibitor l-NAME (100 μM) (C), l-NAME + NMDA (D), PE (1 μM) (E), and PE + NMDA (F) incubated for 30 min before DAF (10 μM) addition. Sections were incubated at 37°C with DAF-FM. D, bar graphs showing DAF-FM fluorescence intensity; data were normalized to vehicle. *, P < 0.05 versus saline (vehicle). #, P < 0.05 versus NMDA.