Figure 3.

Mesenchymal stem cell-specific Alms1 knockout mice show hepatomegaly despite the absence of Cre-driven loss of Alms1 in hepatocytes, while fat pad mass is sexually dimorphic. (A–H) Mass of inguinal, and gonadal white adipose tissue (iWAT and gWAT respectively), liver and interscapular brown adipose tissue (iBAT) of male and female mice at 24 weeks of age. (I–L) Linear regression of serum leptin concentration and lean mass of male and female mice at 24 weeks of age. Each data point represents an individual animal, with bars in (A–H) representing mean ± sd lines and lines in (I–L) representing lines of best fit. Comparison between WT and KO groups in (A–H) used an unpaired two-tailed Student's t-test with Bonferroni correction. Comparison between lines of best fit in (I–L) was performed by simple linear regression, with square brackets showing comparison of y intercepts. No significant change was seen between gradients. ∗ denotes p < 0.05, ∗∗ denotes p < 0.01, ∗∗∗ denotes p < 0.001 and ∗∗∗∗ denotes p < 0.0001. For females N = 7, 8, 8 and 8 for global WT, global KO, MSC WT and MSC KO respectively. For males N = 8, 8, 7 and 7 for global WT, global KO, MSC WT and MSC KO respectively. ns = not significant.