Fig. 3.
Inhibition of Gq/11/PI-PLC signaling pathway of [Ca2+]e- or [Sr2+]e-mediated calcitonin secretion in 6-23 cells. The inhibition of the Gq/11/PI-PLC signaling pathway was achieved by 10-min preincubation of two PI-PLC inhibitors, U73122 and Et-18-OCH3, and one IP3 receptor inhibitor, 2-APB, before cation stimulation. Responses are normalized to the calcitonin secretory response at 10 mM cation. Statistical comparisons of the values were performed by using a one-way ANOVA of each condition, and Dunnett's post-tests were used to compare DMSO control response for each cation to the inhibitors' responses for each cation (n = 6). ***, p < 0.001; **, p < 0.01; *, p < 0.05.