Fig. 4.
Inhibition of [Ca2+]e- or [Sr2+]e-stimulated calcitonin secretion by molecules regulating [Ca2+]i and PC-PLC. The inhibition of [Ca2+]i was achieved by 10-min preincubation of BAPTA-AM before cation stimulation. The inhibition of L-type calcium channel was achieved by 10-min preincubation of nimodipine before cation stimulation. The inhibition of PC-PLC signaling pathway was achieved by 10-min preincubation of D609 before cation stimulation. Responses are normalized to the calcitonin secretory response at 10 mM cation. Statistical comparisons of the values were performed by using a one-way ANOVA of each condition, and Dunnett's post-tests were used to compare DMSO control response for each cation to the inhibitors' responses for each cation (n = 6). ***, p < 0.001.