TABLE 4.
Agonist potencies (EC50) and maximal inhibition and responses (Imax/Rmax) for forskolin-stimulated cAMP production and ERK1/2 phosphorylation
Potencies are in μM for all assays. Imax/Rmax levels are in nM for cAMP inhibition, and fold over basal ERK1/2 response for the ERK1/2 assay.
| Assay | [Ca2+]e |
[Sr2+]e |
||
|---|---|---|---|---|
| Imax or Rmax ± S.E.M. | EC50 (pEC50 ± S.E.M.) | Imax or Rmax ± S.E.M. | EC50 (pEC50 ± S.E.M.) | |
| cAMP | N.A. | N.A. | 65 ± 2 | 339 (3.47 ± 0.05) |
| ERK1/2 | 3.8 ± 0.4 | 3040 (2.52 ± 0.06) | 3.2 ± 0.2 | 405 (3.39 ± 0.14) |
N.A., no activity.