FIG. 4.

Ad-mediated gene transfer to various human cell lines. 293 (A), HUVEC (B), or RD (C) cells preincubated for 10 min at room temperature in either DMEM/F12 or DMEM/F12 containing recombinant Ad5 fiber knob at 100 μg/ml were then exposed for 30 min at room temperature to AdCMVLuc or Ad5lucRGD in DMEM/F12 at 1, 10, or 100 PFU/cell. The unbound virus was aspirated, and complete medium was added. After incubation at 37°C for 30 h, the cells were lysed and the luciferase activity (in relative light units) was determined. Background luciferase activities detected in mock-infected cells were 261, 223, and 163 relative light units for 293, HUVEC, and RD cells, respectively. These activities were subtracted from all readings obtained with the corresponding cell line. Each point represents the mean ± standard deviation of three determinations.