FIG. 5.

Comparison of binding of ¹²⁵I-labeled adenoviruses to 293, HUVEC, or RD cells. One-hundred-microliter aliquots of cells in DMEM-Ad medium (DMEM, 20 mM HEPES, 0.5% BSA), 10⁶ cells per aliquot, were incubated for 1 h at 4°C with 50 μl of ¹²⁵I-labeled Ad (10⁵ cpm per sample). The samples were then diluted with 4 ml of phosphate-buffered saline containing 0.1% BSA, and the cells were pelleted by centrifugation. Radioactivities of cell pellets were determined with a gamma counter. Data shown are means ± standard deviations from an experiment performed in triplicate.