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. 2024 Apr 1;12(4):776. doi: 10.3390/biomedicines12040776

Figure 2.

Figure 2

IgG-T-TCE induced T cell activation. (a,b) Expression of T cell activation markers CD69 and CD25 after 72 h co-culture of PBMCs and positive tumor cells (U266) (E:T, 4:1) in the presence of IgG-T-TCE-NY, measured with FACS. Both CD69 and CD25 expression on T cells increased with the increased concentration of IgG-T-TCE-NY. The colors in the density map represent the cell density at the same location. The cell density increases from blue to red. CD3, FITC; CD69, pacific blue; CD25, APC. (c) Analysis of IL-2 secretion after 72 h co-culture (positive tumor cell line: A375). (d) Analysis of T cell proliferation after 96 h co-culture (positive tumor cell: A375-NY-GFP). CFSE was used to label PBMC before co-culture. The proportion of proliferated T cells to T cells significantly increased with the increase in IgG-T-TCE-NY concentration. CD3, APC. (e) Analysis of granzyme B+ CD8+ T cells after 48 h co-culture (positive tumor cell line: A375). The proportion of granzyme B+ T cells to CD8+ T cells significantly increased with the increase in IgG-T-TCE-NY concentration. Granzyme B, PE; CD8, APC. (f) Analysis of granzyme B+ tumor cells after 48 h co-culture (positive tumor cell line: A375-NY-GFP). The proportion of granzyme B+ tumor cells significantly increased with the increase in IgG-T-TCE-NY concentration. Granzyme B, PE. Data are means ± SD, n = 3.