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. 2024 Mar 27;14(4):405. doi: 10.3390/biom14040405

Figure 4.

Figure 4

Reduced viability of human DPSC-Schwann cells after chloroquine stimulation. (A) Alamar blue assay showing significantly reduced viability in DPSC-derived Schwann cells, starting after 5 h of chloroquine stimulation, remaining observable even after 24 h. (B) Propidium iodide assay illustrating a non-significant decrease in cell survival after 24 h of chloroquine stimulation compared to control cells. (C) Caspase-3 live imaging, illustrating Schwann cell apoptosis starting from 20 h of chloroquine stimulation (20 µM). Data are presented as mean ± SEM. (A): n = Two individual experiments, with four technical replicates in each. (B): n = Three individual experiments with four technical replicates in each. (C): n = One individual experiment with four replicates. Samples were compared with controls at the same timepoint. (A): Mann–Whitney test, (B): Unpaired t-test, and (C): Two-way ANOVA Dunnett test. ** p < 0.001, and *** p < 0.0001. CQ: chloroquine, PI: Propidium Iodide assay, and AB: Alamar Blue assay.