TABLE 2.
Time course of expression of early (p41) and late (gp116) HHV-6 antigens and AL appearance in HSB-2 cells following viral infection
| Time (h) postinfectiona | Mean % of positive cells ± SEMc
|
Mean % of cell sections with AL ± SEMb | |
|---|---|---|---|
| p41 | gp116 | ||
| 0 | 0 | 0 | 0 |
| 24 | 0 | 0 | 0 |
| 48 | 3 ± 0.6 | <1 | 0 |
| 72 | 7 ± 1.4 | 2 ± 0.4 | 1 ± 0.2 |
| 96 | 15 ± 2.5 | 3.5 ± 0.7 | 6 ± 1.2 |
| 108 | 30 ± 4.2 | 8 ± 1.2 | 11 ± 2.7 |
As assessed by indirect immunofluorescence.
Percentage of cell sections in which AL were present at EM observation.
For each time point postinfection, 1,000 cells were randomly analyzed by immunofluorescence assay and 50 cell sections (15 to 20 μm in diameter and crossing the nucleus) were randomly analyzed by EM. The results are means of three different experiments.