Figure 5.

Proliferation by Ki67 staining is increased with NTCU, advancing histology, and in XPC KO. (A) Representative immunofluorescence microscopy image of a large airway showing co-localization of Ki67 and CK5/6 (orange and yellow squares with corresponding regions of interest blown up). 20× magnification. (B) Representative images of Ki67 staining by immunohistochemistry in XPC WT and KO bronchi by histology. Note the increase in Ki67+ cells in the basal epithelium of the airways. (C) Quantification of % Ki67+ cells by treatment (NTCU vs. acetone), XPC expression (XPC WT and KO), and histology. LG = low-grade dysplasia. HG = high-grade dysplasia. LUSC = lung squamous cell carcinoma. * p < 0.05, ** p < 0.01, *** p < 0.001 using two-way ANOVA.