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. 1998 Dec;72(12):9755–9762. doi: 10.1128/jvi.72.12.9755-9762.1998

FIG. 1.

FIG. 1

Expression and subcellular localization of deletion mutants of BDVP. (A) The cDNA fragments of BDV P inserted into pcDL-HA are shown. The numbers indicate amino acid positions of BDV P. (B) Expression of wild-type and deletion mutants of BDV P in COS-7 cells as detected by Western blotting with rabbit antiserum. (C) IFA staining of COS-7 cells expressing wild-type and deletion mutants of BDV P with rabbit anti-BDV P. Noninfected MDCK cells (MDCK/−) served as a negative control (a), and MDCK/BDV cells served as a positive control of BDV P in persistently infected cells (b). COS-7 cells were transfected with pCDL-HA (c), pP-Wild (e), pP-del.N18 (f), pP-del.N40 (g), pP-del.N82 (h), pP-del.C117 (i), pP-del.C148 (j), or pP-del.C182 (k). Nontransfected COS-7 cells (COS7/−) served as a negative transfection control (d).