FIG. 2.

Expression and subcellular localization of β-galactosidase fused with the N-terminal, C-terminal, and middle regions of BDV P. (A) Fragments of BDV P fused to β-galactosidase are shown schematically. The numbers indicate the amino acid positions of BDV P. (B) Expression of the fusion protein in COS-7 cells as detected by Western blotting with mouse anti-β-galactosidase MAb. (C) IFA staining of COS-7 cells expressing fusion proteins with anti-β-galactosidase MAb. COS-7 cells were transfected with pRSV-LacZ (a), pRSV-HA (b), pRSV-1/41 (d), pRSV-41/181 (e), or pRSV-172/201 (f). Nontransfected COS-7 cells (COS7/−) served as a negative control (c).