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. 1998 Dec;72(12):9763–9770. doi: 10.1128/jvi.72.12.9763-9770.1998

FIG. 2.

FIG. 2

Effects of the V3 peptides (V3-BH10 and CTR36) corresponding to the V3 region of HIV-1 IIIB (A), V3-ELI corresponding to that of T-tropic HIV-1 strain ELI (B), V3-89.6 corresponding to that of dualtropic HIV-1 strain 89.6 (C), or V3-ADA corresponding to that of M-tropic HIV-1 strain ADA (D) on the staining of biotinylated IVR7. JY cells were incubated with human IgG on ice for 15 min to block nonspecific binding. Cells were then preincubated with various concentrations (15 μM [dashed line], 5 μM [dashed and dotted line], 1.7 μM [dotted line], and 0 μM [solid line]) of V3-BH10, control linear V3 peptide (CTR36), V3-ELI, V3-89.6, or V3-ADA on ice for 30 min. Then, biotinylated IVR7 or FITC-Leu12 was added. After being washed, cells were further incubated with PE-conjugated streptavidin on ice, washed, and subjected to flow cytometric analysis. Reactivity was determined by comparison of results of the control staining with those with biotinylated irrelevant mouse IgG1 or FITC-conjugated irrelevant mouse IgG (cont. IgG1 or cont. IgG, respectively).