FIG. 4.

Effects of the V3 peptides on HIV-1 infection in an MTT assay. One-hundred microliters of the cell suspension of MT-4 cells was added to each well. Then, various concentrations of the indicated synthetic peptides were added to each well and incubated for 30 min at 37°C. After that, HIV-1 solution (HIV-1 IIIB strain) was further added to each well at a multiplicity of infection of 0.0008. After a 5-day incubation, the cell viability was determined by the MTT method. Anti-HIV-1 activity in vitro of the five synthesized V3 peptides was analyzed by inhibition of virus-induced cell death. Values are means and are expressed as the arithmetic means ± standard deviations.