TABLE 1.
Inhibition of biochemical activities of SV40 T antigen by T-antigen-specific monoclonal antibodies
| Antibody | Inhibition ofa:
|
||||||
|---|---|---|---|---|---|---|---|
| SV40 DNA binding | ATPase | Helicase | Unwinding
|
Complex formation
|
|||
| Linear | CCS | RPA | Pol/Prim | ||||
| 108 | − | − | − | − | − | − | − |
| 419 | − | − | − | − | + | − | − |
| 416 | − | − | − | p | + | − | − |
| 220 | − | − | − | p | p | + | − |
| 221 | − | − | − | p | p | + | − |
| 204 | + | + | + | + | + | + | + |
| 414 | − | − | + | + | + | − | + |
| 101 | − | − | − | − | − | − | − |
| KT3 | − | − | − | − | − | − | − |
a
Purified monoclonal antibodies were tested for the ability to inhibit SV40 origin DNA binding (Fig. 4A); ATPase activity (Fig. 4B); helicase activity using an oligonucleotide-primed M13mp18 ssDNA (Fig. 4C); SV40 origin DNA unwinding activity on linear and closed circular supercoiled (CCS) templates (Fig. 4D and E and data not shown); and complex formation with RPA and Pol/Prim (Fig. 3). +, inhibition; p, partial inhibition; −, lack of inhibition.