Figure 5.

Size exclusion chromatography of <3 kDa from protease_SE5 (a) and Alcalase (b). Each fraction (2.5 mL) was eluted by deionized water at a flow rate of 23 mL/h. The active fractions were lyophilized before analysis by LC-MS/MS and de novo peptide sequencing. Antioxidant activity of active fractions from protease_SE5 (c) and Alcalase (d) obtained from the Sephadex G-25 column. The experiments are performed in triplicate (n = 3). Different letters (a–d) indicate significant differences at p  <  0.05 according to analysis by Duncan’s multiple range test.