Table 3.
List of identified peptides from CS protein hydrolysate by LC-MS/MS coupled with de novo sequencing.
| Peptide Sequence | ALC (%) * | Local Confidence (%) | m/z | Molecular Weight (Da) |
|---|---|---|---|---|
| Protease_SE5 | ||||
| Fraction 3 | ||||
| FLGY | 90 | 93 84 88 93 | 499.2525 | 498.2478 |
| FGGGF | 94 | 92 86 96 97 98 | 484.2185 | 483.2118 |
| FDYGKY | 98 | 97 98 98 95 98 99 | 396.6815 | 791.3489 |
| FYDTYY | 93 | 80 89 96 96 99 99 | 436.1788 | 870.3436 |
| YSYAYDDR | 99 | 98 99 99 99 99 98 98 99 | 526.7186 | 1051.4246 |
| Fraction 4 | ||||
| YTRPY | 98 | 99 99 99 98 97 | 350.1793 | 698.3387 |
| YTEYAF | 96 | 90 92 98 97 99 99 | 397.1747 | 792.3330 |
| FDFVWVQ | 96 | 92 95 79 83 99 99 99 | 397.1747 | 792.3330 |
| Alcalase | ||||
| Fraction 3 | ||||
| YTDHGAF | 98 | 99 99 99 98 97 98 99 | 405.6722 | 809.3344 |
| FGGGGSFPP | 91 | 96 93 99 99 98 99 82 62 90 | 411.6886 | 821.3708 |
| Fraction 4 | ||||
| FGGY | 91 | 95 86 90 93 | 443.1905 | 442.1852 |
| FDYLR | 93 | 94 96 93 90 93 | 357.1833 | 712.3544 |
| DYFYY | 92 | 88 81 95 97 99 | 385.6546 | 769.2959 |
| WDAFHPT | 93 | 89 93 95 98 97 92 88 | 437.1962 | 872.3817 |
* ALC (%) represents average local confidence.