Table 4.
Percentage of key antioxidative amino acids, prediction of biological activity, and toxicity of peptides identified from protease_SE5- and Alcalase-derived of CS protein hydrolysate.
| Peptide Sequence | Key Constituent Antioxidative Amino Acids (%) | Peptide Ranker * | BIOPEP | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Cys | His | Met | Phe | Trp | Tyr | Biological Activity ** | Toxicity *** | ||
| Protease_SE5 | |||||||||
| Fraction 3 | |||||||||
| FLGY | 0 | 0 | 0 | 16.7 | 0 | 16.7 | 0.91 | ACE inhibitor, dipeptidyl peptidase IV inhibitor | Non-toxic |
| FGGGF | 0 | 0 | 0 | 33.4 | 0 | 0 | 0.98 | ACE inhibitor, dipeptidyl peptidase IV inhibitor | Non-toxic |
| FDYGKY | 0 | 0 | 0 | 16.7 | 0 | 33.4 | 0.67 | ACE inhibitor | Non-toxic |
| FYDTYY | 0 | 0 | 0 | 16.7 | 0 | 33.4 | 0.60 | ACE inhibitor, dipeptidyl peptidase IV inhibitor | Non-toxic |
| Fraction 4 | |||||||||
| FDFVWVQ | 0 | 0 | 0 | 33.4 | 16.7 | 0 | 0.68 | ACE inhibitor, dipeptidyl peptidase IV inhibitor, antioxidant | Non-toxic |
| Alcalase | |||||||||
| Fraction 3 | |||||||||
| YTDHGAF | 0 | 16.7 | 0 | 16.7 | 0 | 16.7 | 0.40 | dipeptidyl peptidase IV inhibitor | Non-toxic |
| FGGGGSFPP | 0 | 0 | 0 | 33.4 | 0 | 0 | 0.84 | ACE inhibitor, dipeptidyl peptidase IV inhibitor | Non-toxic |
| Fraction 4 | |||||||||
| FGGY | 0 | 0 | 0 | 16.7 | 0 | 16.7 | 0.95 | ACE inhibitor | Non-toxic |
| FDYLR | 0 | 0 | 0 | 16.7 | 0 | 16.7 | 0.81 | ACE inhibitor | Non-toxic |
| DYFYY | 0 | 0 | 0 | 16.7 | 0 | 50.1 | 0.84 | ACE inhibitor, antioxidant | Non-toxic |
| WDAFHPT | 0 | 16.7 | 0 | 16.7 | 16.7 | 0 | 0.84 | ACE inhibitor, dipeptidyl peptidase IV inhibitor | Non-toxic |
* Prediction of the probability that the peptide will be bioactive, available at http://distilldeep.ucd.ie/PeptideRanker/ (accessed on 23 February 2024). ** Prediction of the biological activity by BIOPEP, available at http://www.uwm.edu.pl/biochemia/index.php/pl/biopep (accessed on 23 February 2024). *** Prediction of toxicity of peptides by ToxinPred, available at https://webs.iiitd.edu.in/raghava/toxinpred/index.html (accessed on 23 February 2024).